Super-resolution optical imaging from STED to MINFLUX
1.Institute of Photonic Chips University of Shanghai for Science and Technology, Shanghai, China
2.Centre for Artificial-Intelligence Nanophotonics, School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, China
KEYWORDS: STED, microscopy, MINFLUX, super resolution
Stimulated emission depletion (STED) microscopy has matured into a powerful and widely used super-resolution imaging method. MINFLUX which stands for minimal photon fluxes，is the next revolutionary fluorescence microscope with a spatial resolution around 1 nm in fixed and living cells. In particular, MINFLUX use a donut-shaped excitation beam whose central zero is targeted to pre-defined positions close to the fluorophore. The fluorophore position can thus be determined with a minimal number of photons and consequently within a spatio-temporal regime that exceeds alternative techniques.
This talk will highlight the new development of STED microscopy in our lab. We will also present the recent progress of our research group on MINFLUX imaging. Super-resolution imaging of specific synaptic proteins obtained for the first time. Moreover, quantum dots-assisted MINFLUX microscopy has great potential to investigate microstructures that require super-resolution and long-term imaging. We amply demonstrate that MINFLUX will allow researchers to address numerous biomedical and biophysical questions on the molecular scale. Based on the above results, we will outlook the role of MINFLUX in the field of artificial intelligence in the future.
Professor Gu is Executive Chancellor of the University Council and Distinguished Professor of University of Shanghai for Science and Technology. He was Distinguished Professor and Associate Deputy Vice-Chancellor at RMIT University and a Laureate Fellow of the Australian Research Council. He is an author of four standard reference books and has over 500 publications in nano/biophotonics. He is an elected Fellow of the Australian Academy of Science and the Australian Academy of Technological Sciences and Engineering as well as Foreign Fellow of the Chinese Academy of Engineering. He is also an elected fellow of the AIP, the OSA, the SPIE, the InstP, and the IEEE. He was President of the International Society of Optics within Life Sciences, Vice President of the Board of the International Commission for Optics (ICO) (Chair of the ICO Prize Committee) and a Director of the Board of the Optical Society of America (Chair of the International Council). He was awarded the Einstein Professorship, the W. H. (Beattie) Steel Medal, the Ian Wark Medal, the Boas Medal and the Victoria Prize for Science and Innovation. Professor Gu is a winner of the 2019 Dennis Gabor Award of SPIE.